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N.C. Department of Environment and Natural Resources

NC Department of Environment and Natural Resources
Water Quality - ATB General Information

Water Quality

Aquatic Toxicity Testing

Aquatic Toxicology Branch(ATB)

Unit Main Number: 919-743-8401

 

 

ATB Programs and General Information

The Aquatic Toxicology Branch (ATB) serves the state of North Carolina through its support of the EPA mandated NPDES program as it pertains to the Clean Water Act (CWA) established in 1972.  ATB performs three main functions in support of this program:

  • NPDES Compliance and Enforcement

All permitted dischargers of complex wastewaters in the state of North Carolina are required to perform self-monitoring of aquatic toxicity in their wastewater.  Currently, there are over 500 industrial and municipal facilities in North Carolina.  The Aquatic Toxicology Branch reviews all toxicity data reported by these facilities to verify data quality, track compliance with established permit limits and make enforcement recommendations for non-compliant situations.

  • Biological Laboratory Certification and Inspection

All toxicity analyses reported by dischargers must, by water quality regulations, be performed by a biological laboratory certified by the state of North Carolina.  The Aquatic Toxicology Branch operates this certification program which includes laboratory inspections, data tracking and performance evaluation testing.

  • Whole Effluent Toxicity (WET) Testing and Bioassay Organism Culture

The Aquatic Toxicology Unit (ATB) Testing Laboratory conducts toxicity testing to support the Whole Effluent Toxicity (WET) monitoring program and various special toxicity studies. 

ATB uses modified EPA methods for measuring the acute and chronic toxicity of wastewater and surface waters to freshwater and marine organisms.  The principle organisms used for WET testing are Ceriodaphnia dubia (water fleas), Pimephales promelas (fathead minnows) and Mysidopsis bahia (mysid shrimp).

The ATB Culture Laboratory maintains in-house Ceriodaphnia dubia cultures for WET testing and Daphnia pulex cultures for special toxicity studies.

 

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